Adenosine and adenosine-5′-monophosphate ingestion ameliorates abnormal glucose metabolism in mice fed a high-fat diet

Ardiansyah, Ardiansyah and Inagawa, Yuto and Koseki, Takuya and Agista, Afifah Zahra and Ikeda, Ikuo and Goto, Tomoko and Komai, Michio (2018) Adenosine and adenosine-5′-monophosphate ingestion ameliorates abnormal glucose metabolism in mice fed a high-fat diet. BMC Complementary and Alternative Medicine, 18 (304). ISSN 1472-6882

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Official URL: https://link.springer.com/article/10.1186/s12906-0...

Abstract

Background We have previously reported that ingestion of adenosine (ADN) and adenosine-5′-monophosphate (AMP) improves abnormal glucose metabolism in the stroke-prone spontaneously hypertensive rat model of non-obesity-associated insulin resistance. In this study, we investigated the effect of ADN and AMP ingestion on glucose metabolism in mice with high-fat diet-induced obesity. Methods Seven-week-old C57BL/6 J mice were administered distilled water (as a control), 10 mg/L ADN, or 13 mg/L AMP via their drinking water for 14 or 25 weeks, during which they were fed a high-fat diet. Oral glucose tolerance test (OGTT) was conducted on 21-week-old mice fasted for 16 h. Insulin tolerance test (ITT) was performed on 22-week-old mice fasted for 3 h. Blood and muscle were collected for further analysis of serum parameters, gene and protein expression levels, respectively. Results Glucose metabolism in the ADN and AMP groups was significantly improved compared with the control. OGTT and ITT showed that ADN and AMP groups lower than control group. Furthermore, phosphorylation of AMP-activated protein kinase (AMPK) and mRNA levels of genes involved in lipid oxidation were enhanced in the skeletal muscle of ADN- and AMP-treated mice. Conclusion These results indicate that ingestion of ADN or AMP induces activation of AMPK in skeletal muscle and mitigates insulin resistance in mice with high-fat diet-induced diabetes.

Item Type: Article
Uncontrolled Keywords: Adenosine Adenosine-5′-monophosphate Glucose metabolism High-fat diet
Subjects: Food Science and Technology
Divisions: Fakultas Teknik dan Ilmu Komputer > Program Studi Ilmu dan Teknologi Pangan
Depositing User: Ahmad Yani
Date Deposited: 23 Nov 2018 09:40
Last Modified: 23 Nov 2018 09:40
URI: http://repository.bakrie.ac.id/id/eprint/2094

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